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RUVBL proteins are involved in plant gametophyte development
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SYSNO ASEP 0571955 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title RUVBL proteins are involved in plant gametophyte development Author(s) Tomaštíková, Eva (UEB-Q) RID, ORCID
Yang, Fen (UEB-Q) ORCID
Mlynárová, L. (SK)
Hafidh, Said (UEB-Q) RID, ORCID
Schořová, Š. (CZ)
Kusová, A. (CZ)
Pernisová, M. (CZ)
Přerovská, T. (CZ)
Klodová, Božena (UEB-Q) ORCID
Honys, David (UEB-Q) RID, ORCID
Fajkus, Jiří (BFU-R) RID, ORCID
Pečinka, Aleš (UEB-Q) ORCID, RID
Schrumpfová, P. (CZ)Number of authors 13 Source Title Plant Journal. - : Wiley - ISSN 0960-7412
Roč. 114, č. 2 (2023), s. 325-337Number of pages 13 s. Language eng - English Country US - United States Keywords MULTIPLE SEQUENCE ALIGNMENT ; TELOMERE-BINDING-PROTEIN ; ARABIDOPSIS OECD category Plant sciences, botany R&D Projects GA21-15841S GA ČR - Czech Science Foundation (CSF) GX20-01331X GA ČR - Czech Science Foundation (CSF) GA22-00871S GA ČR - Czech Science Foundation (CSF) GA22-29717S GA ČR - Czech Science Foundation (CSF) LM2018129 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 ; BFU-R - RVO:68081707 UT WOS 000940668400001 EID SCOPUS 85149783516 DOI 10.1111/tpj.16136 Annotation The proper development of male and female gametophytes is critical for successful sexual reproduction and requires a carefully regulated series of events orchestrated by a suite of various proteins. RUVBL1 and RUVBL2, plant orthologues of human Pontin and Reptin, respectively, belong to the evolutionarily highly conserved AAA+ family linked to a wide range of cellular processes. Previously, we found that RUVBL1 and RUVBL2A mutations are homozygous lethal in Arabidopsis. Here, we report that RUVBL1 and RUVBL2A play roles in reproductive development. We show that mutant plants produce embryo sacs with an abnormal structure or with various numbers of nuclei. Although pollen grains of heterozygous mutant plants exhibit reduced viability and reduced pollen tube growth in vitro, some of the ruvbl pollen tubes are capable of targeting ovules in vivo. Similarly, some ruvbl ovules retain the ability to attract wild-type pollen tubes but fail to develop further. The activity of the RUVBL1 and RUVBL2A promoters was observed in the embryo sac, pollen grains, and tapetum cells and, for RUVBL2A, also in developing ovules. In summary, we show that the RUVBL proteins are essential for the proper development of both male and particularly female gametophytes in Arabidopsis. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2024 Electronic address https://doi.org/10.1111/tpj.16136
Number of the records: 1