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The beta subunit of nascent polypeptide associated complex plays a role in flowers and siliques development of arabidopsis thaliana
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SYSNO ASEP 0524246 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title The beta subunit of nascent polypeptide associated complex plays a role in flowers and siliques development of arabidopsis thaliana Author(s) Fíla, Jan (UEB-Q) RID, ORCID
Klodová, Božena (UEB-Q) ORCID
Potěšil, D. (CZ)
Juříček, Miloslav (UEB-Q) RID, ORCID
Šesták, Petr (UEB-Q) ORCID
Zdráhal, Z. (CZ)
Honys, David (UEB-Q) RID, ORCIDNumber of authors 7 Article number 2065 Source Title International Journal of Molecular Sciences. - : MDPI
Roč. 21, č. 6 (2020)Number of pages 31 s. Language eng - English Country CH - Switzerland Keywords Arabidopsis thaliana ; Chaperone ; Flower bud proteome ; Flower bud transcriptome ; Male gametophyte ; Nascent polypeptide-associated complex Subject RIV EF - Botanics OECD category Plant sciences, botany R&D Projects LTC18043 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA18-02448S GA ČR - Czech Science Foundation (CSF) GA19-01723S GA ČR - Czech Science Foundation (CSF) EF16_019/0000738 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) Method of publishing Open access Institutional support UEB-Q - RVO:61389030 UT WOS 000529890200161 EID SCOPUS 85082085747 DOI 10.3390/ijms21062065 Annotation The nascent polypeptide-associated (NAC) complex was described in yeast as a heterodimer composed of two subunits, α and β, and was shown to bind to the nascent polypeptides newly emerging from the ribosomes. NAC function was widely described in yeast and several information are also available about its role in plants. The knock down of individual NAC subunit(s) led usually to a higher sensitivity to stress. In Arabidopsis thaliana genome, there are five genes encoding NACα subunit, and two genes encoding NACβ. Double homozygous mutant in both genes coding for NACβ was acquired, which showed a delayed development compared to the wild type, had abnormal number of flower organs, shorter siliques and greatly reduced seed set. Both NACβ genes were characterized in more detail—the phenotype of the double homozygous mutant was complemented by a functional NACβ copy. Then, both NACβ genes were localized to nuclei and cytoplasm and their promoters were active in many organs (leaves, cauline leaves, flowers, pollen grains, and siliques together with seeds). Since flowers were the most affected organs by nacβ mutation, the flower buds’ transcriptome was identified by RNA sequencing, and their proteome by gel-free approach. The differential expression analyses of transcriptomic and proteomic datasets suggest the involvement of NACβ subunits in stress responses, male gametophyte development, and photosynthesis. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2021 Electronic address http://doi.org/10.3390/ijms21062065
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