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Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L
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SYSNO ASEP 0346619 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L Author(s) Li, P. (CN)
Hulak, M. (CZ)
Koubek, Pavel (BTO-N)
Šulc, Miroslav (MBU-M) RID, ORCID
Dzyuba, B. (UA)
Boryshpolets, S. (UA)
Rodina, M. (CZ)
Gela, D. (CZ)
Maňásková-Postlerová, Pavla (BTO-N) ORCID
Pěknicová, Jana (BTO-N) RID
Linhart, O. (CZ)Number of authors 11 Source Title Theriogenelogy. - : Elsevier - ISSN 0093-691X
Roč. 74, č. 3 (2010), s. 413-423Number of pages 11 s. Language eng - English Country US - United States Keywords sperm quality ; cryodamage ; spermatozoa ; seminal plasma ; fish Subject RIV EI - Biotechnology ; Bionics CEZ AV0Z50520701 - BTO-N (2007-2013) AV0Z5020903 - MBU-M UT WOS 000280341300009 DOI 10.1016/j.theriogenology.2010.02.024 Annotation Damage to spermatozoa during cryopreservation is regarded as a major obstacle to the expansion of sperm storage technology. The authors used 2D PAA gel electrophoresis and MALDI-TOF to explore whether the protein profile of common carp (Cyprinus carpio) spermatozoa is affected by cryopreservation. 14 protein spots were significantly altered following cryopreservation. Eleven of these were identified: three as specific membrane proteins involved in membrane trafficking, organization, and cell movement; six as cytoplasmic enzymes involved in cell metabolism, oxidoreductase activity, and signal transduction; and transferrin. Based on these findings, the authors hypothesize that transferrin in cryopreserved sperm may protect spermatozoa against oxidative damage during the freeze-thaw process. Cryopreservation caused changes in spermatozoa protein profiles that may lead to decreased spermatozoa velocity, motility, fertilization success and to subsequent ova hatching rate. Workplace Institute of Biotechnology Contact Monika Kopřivová, Monika.Koprivova@ibt.cas.cz, Tel.: 325 873 700 Year of Publishing 2011
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