Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L

0346619 - BTÚ 2011 RIV US eng J - Journal Article
Li, P. - Hulak, M. - Koubek, Pavel - Šulc, Miroslav - Dzyuba, B. - Boryshpolets, S. - Rodina, M. - Gela, D. - Maňásková-Postlerová, Pavla - Pěknicová, Jana - Linhart, O.
Ice-age endurance: the effects of cryopreservation on proteins of sperm of common carp, Cyprinus carpio L.
Theriogenelogy. Roč. 74, č. 3 (2010), s. 413-423. ISSN 0093-691X. E-ISSN 1879-3231
Institutional research plan: CEZ:AV0Z50520701; CEZ:AV0Z5020903
Keywords : sperm quality * cryodamage * spermatozoa * seminal plasma * fish
Subject RIV: EI - Biotechnology ; Bionics
Impact factor: 2.045, year: 2010

Damage to spermatozoa during cryopreservation is regarded as a major obstacle to the expansion of sperm storage technology. The authors used 2D PAA gel electrophoresis and MALDI-TOF to explore whether the protein profile of common carp (Cyprinus carpio) spermatozoa is affected by cryopreservation. 14 protein spots were significantly altered following cryopreservation. Eleven of these were identified: three as specific membrane proteins involved in membrane trafficking, organization, and cell movement; six as cytoplasmic enzymes involved in cell metabolism, oxidoreductase activity, and signal transduction; and transferrin. Based on these findings, the authors hypothesize that transferrin in cryopreserved sperm may protect spermatozoa against oxidative damage during the freeze-thaw process. Cryopreservation caused changes in spermatozoa protein profiles that may lead to decreased spermatozoa velocity, motility, fertilization success and to subsequent ova hatching rate.
Permanent Link: http://hdl.handle.net/11104/0187599