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Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic
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SYSNO ASEP 0334916 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic Author(s) Kváč, Martin (BC-A) RID, RID, ORCID
Sak, Bohumil (BC-A) RID, ORCID
Hanzlíková, D. (CZ)
Kotilová, J. (CZ)
Květoňová, Dana (BC-A) SAISource Title Parasitology Research. - : Springer - ISSN 0932-0113
Roč. 104, č. 2 (2009), s. 425-428Number of pages 4 s. Language eng - English Country DE - Germany Keywords Cryptosporidium spp. ; slaughterhouse ; pigs Subject RIV GJ - Animal Vermins ; Diseases, Veterinary Medicine R&D Projects GP523/07/P117 GA ČR - Czech Science Foundation (CSF) CEZ AV0Z60220518 - PAU-O, BC-A (2005-2011) UT WOS 000262652600031 Annotation A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial SSU rRNA and GP60 partial genes as well as PCR-RFLP of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and 2 sows (10%). Twenty one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and C. suis in finishers were found. Both sows were infected with the C. parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time. Workplace Biology Centre (since 2006) Contact Dana Hypšová, eje@eje.cz, Tel.: 387 775 214 Year of Publishing 2010
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