Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic

0334916 - BC 2010 RIV DE eng J - Journal Article
Kváč, Martin - Sak, Bohumil - Hanzlíková, D. - Kotilová, J. - Květoňová, Dana
Molecular characterization of Cryptosporidium isolates from pigs at slaughterhouses in South Bohemia, Czech Republic.
Parasitology Research. Roč. 104, č. 2 (2009), s. 425-428. ISSN 0932-0113. E-ISSN 1432-1955
R&D Projects: GA ČR GP523/07/P117
Institutional research plan: CEZ:AV0Z60220518
Keywords : Cryptosporidium spp. * slaughterhouse * pigs
Subject RIV: GJ - Animal Vermins ; Diseases, Veterinary Medicine
Impact factor: 1.721, year: 2009

A total of 123 fecal samples of slaughtered finisher pigs and 21 sows from 14 farms were screened for Cryptosporidium spp. infection using the aniline-carbol-methyl violet staining method. Positive samples were molecularly characterized by direct sequencing of partial SSU rRNA and GP60 partial genes as well as PCR-RFLP of SSU rRNA. Cryptosporidium oocysts were microscopically identified in 36 finishers (29%) and 2 sows (10%). Twenty one mono-infections of Cryptosporidium pig genotype II and 15 mixed-infection of Cryptosporidium pig genotype II and C. suis in finishers were found. Both sows were infected with the C. parvum subgenotype IIaA16G1R1, which is reported from pigs for the first time.
Permanent Link: http://hdl.handle.net/11104/0179525