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Zebrafish kidney stromal cell lines support multilineage hematopoiesis
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SYSNO ASEP 0333657 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Zebrafish kidney stromal cell lines support multilineage hematopoiesis Author(s) Stachura, D.L. (US)
Reyes, J.R. (US)
Bartůněk, Petr (UMG-J) RID
Paw, B.H. (US)
Zon, L.I. (US)
Traver, D. (US)Number of authors 6 Source Title Blood. - : American Society of Hematology - ISSN 0006-4971
Roč. 114, č. 2 (2009), s. 279-289Number of pages 11 s. Language eng - English Country US - United States Keywords Stromal cell line ; hematopoietic progenitor cells ; zebrafish growth factors Subject RIV EB - Genetics ; Molecular Biology R&D Projects LC06077 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50520514 - UMG-J (2005-2011) UT WOS 000268061700013 DOI 10.1182/blood-2009-02-203638 Annotation To enable more precise analyses of zebrafish hematopoietic progenitor cells, we have created zebrafish kidney stromal (ZKS) cell lines. Culture of kidney marrow with ZKS cells results in the maintenance and expansion of hematopoietic precursor cells. Hematopoietic growth is dependent upon ZKS cells, and we show that ZKS cells express many growth factors previously demonstrated to be important in maintaining mammalian hematopoietic cells. In the absence of exogenous growth factors, ZKS cells maintain early hematopoietic precursors and support differentiation of lymphoid and myeloid cells. With the addition of zebrafish erythropoietin, ZKS cells also support the differentiation of erythroid precursors. The development of robust in vitro assays now provide the means to track defined, functional outcomes for prospectively isolated blood cell subsets in the zebrafish. Workplace Institute of Molecular Genetics Contact Nikol Škňouřilová, nikol.sknourilova@img.cas.cz, Tel.: 241 063 217 Year of Publishing 2010
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