Zebrafish kidney stromal cell lines support multilineage hematopoiesis

0333657 - ÚMG 2010 RIV US eng J - Journal Article
Stachura, D.L. - Reyes, J.R. - Bartůněk, Petr - Paw, B.H. - Zon, L.I. - Traver, D.
Zebrafish kidney stromal cell lines support multilineage hematopoiesis.
Blood. Roč. 114, č. 2 (2009), s. 279-289. ISSN 0006-4971. E-ISSN 1528-0020
R&D Projects: GA MŠMT(CZ) LC06077
Institutional research plan: CEZ:AV0Z50520514
Keywords : Stromal cell line * hematopoietic progenitor cells * zebrafish growth factors
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 10.555, year: 2009

To enable more precise analyses of zebrafish hematopoietic progenitor cells, we have created zebrafish kidney stromal (ZKS) cell lines. Culture of kidney marrow with ZKS cells results in the maintenance and expansion of hematopoietic precursor cells. Hematopoietic growth is dependent upon ZKS cells, and we show that ZKS cells express many growth factors previously demonstrated to be important in maintaining mammalian hematopoietic cells. In the absence of exogenous growth factors, ZKS cells maintain early hematopoietic precursors and support differentiation of lymphoid and myeloid cells. With the addition of zebrafish erythropoietin, ZKS cells also support the differentiation of erythroid precursors. The development of robust in vitro assays now provide the means to track defined, functional outcomes for prospectively isolated blood cell subsets in the zebrafish.
Permanent Link: http://hdl.handle.net/11104/0178596