Sensitive electrochemical detection of native and aggregated alpha-synuclein protein involved in Parkinson's disease

Masařík, Michal; Stobiecka, A.; Kizek, René; Jelen, František; Pechan, Zdeněk; Hoyer, W.; Jovin, T.; Subramaniam, V.; Paleček, Emil

Number of the records: 1

Sensitive electrochemical detection of native and aggregated alpha-synuclein protein involved in Parkinson's disease

1.

SYSNO ASEP	0104692
Document Type	J - Journal Article
R&D Document Type	Journal Article
Subsidiary J	Ostatní články
Title	Sensitive electrochemical detection of native and aggregated alpha-synuclein protein involved in Parkinson's disease
Title	Elektrochemická analýza nativního a agregovaného alfa-synucleinu, proteinu, který se účastní na neurodegenerativních změnách vedoucích k Parkinsonově chorobě
Author(s)	Masařík, Michal (BFU-R) Stobiecka, A. (PL) Kizek, René (BFU-R) Jelen, František (BFU-R)_RID Pechan, Zdeněk (BFU-R) Hoyer, W. (DE) Jovin, T. (DE) Subramaniam, V. (DE) Paleček, Emil (BFU-R)_{RID, ORCID}
Source Title	Electroanalysis. - : Wiley - ISSN 1040-0397 Roč. 16, 13-14 (2004), s. 1172-1181
Number of pages	10 s.
Language	eng - English
Country	US - United States
Keywords	electrochemistry of proteins ; alpha-synuclein aggregation ; adsorptive transfer stripping
Subject RIV	BO - Biophysics
R&D Projects	GA204/03/0566 GA ČR - Czech Science Foundation (CSF)
CEZ	AV0Z5004920 - BFU-R
Annotation	The aggregation of alpha-synuclein, a 14 kDa protein, is involved in several human neurodegenerative disorders, including Parkinson's disease. We studied native and in vitro aggregated alpha-synuclein by circular dichroism (CD), atomic force microscopy (AFM) and electrochemical methods. We used constant current chronopotentiometric stripping analysis (CPSA) to measure hydrogen evolution catalyzed by alpha-synuclein (peak H) at hanging mercury drop electrodes (HMDE) and square-wave stripping voltammetry (SWSV) to monitor tyrosine oxidation at carbon paste electrodes (CPE). To decrease the volume of the analyte, most of the electrochemical measurements were performed by adsorptive transfer (medium exchange) from 3 - 6 muL drops of alpha-synuclein samples.
Workplace	Institute of Biophysics
Contact	Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244
Year of Publishing	2005

Number of the records: 1