Development of a new Sindbis virus transducing system and its characterization in three Culicine mosquitoes and two Lepidopteran species
1.
SYSNO ASEP
0101614
Document Type
J - Journal Article
R&D Document Type
Journal Article
Subsidiary J
Ostatní články
Title
Development of a new Sindbis virus transducing system and its characterization in three Culicine mosquitoes and two Lepidopteran species
Title
Vývoj nového transdukčního systému na bazi viru Sindbis a jeho characterizace u trech druhů komárů (Culicinae) a dvou druhů motýlů (Lepidoptera)
Author(s)
Foy, B. D. (US) Myles, K. M. (US) Pierro, D. J. (US) Sanchez-Vargas, I. (US) Uhlířová, Miroslava (ENTU-I) Jindra, Marek (ENTU-I) Beaty, B. J. (US) Olson, K. E. (US)
Source Title
Insect Molecular Biology. - : Wiley
- ISSN 0962-1075
Roč. 13, č. 1 (2004), s. 89-100
Number of pages
12 s.
Language
eng - English
Country
GB - United Kingdom
Keywords
Sindbis ; alphavirus ; mosquito
Subject RIV
EB - Genetics ; Molecular Biology
CEZ
AV0Z5007907 - ENTU-I, BC-A
Annotation
Following per os infection, a new Sindbis virus 5'dsMRE16ic-EGFP was detected in the gut of Aedes aegypti and Culex tritaeniorhynchus, but not Culex pipiens pipiens. Upon parenteral infection of larvae, the silkworm Bombyx mori showed EGFP expression in larvae, pupae and adults, while the tobacco hornworm Manduca sexta was resistant to the virus. 5'dsMRE16ic should be useful for gene expression in some important insect species that are otherwise difficult to manipulate genetically
Workplace
Institute of Entomology (until 2005)
Contact
Helena Musilová, musilova@entu.cas.cz, Tel.: 387 775 216
Year of Publishing
2005
Number of the records: 1
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