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Development of a new Sindbis virus transducing system and its characterization in three Culicine mosquitoes and two Lepidopteran species
- 1.0101614 - ENTU-I 20043020 RIV GB eng J - Journal Article
Foy, B. D. - Myles, K. M. - Pierro, D. J. - Sanchez-Vargas, I. - Uhlířová, Miroslava - Jindra, Marek - Beaty, B. J. - Olson, K. E.
Development of a new Sindbis virus transducing system and its characterization in three Culicine mosquitoes and two Lepidopteran species.
[Vývoj nového transdukčního systému na bazi viru Sindbis a jeho characterizace u trech druhů komárů (Culicinae) a dvou druhů motýlů (Lepidoptera).]
Insect Molecular Biology. Roč. 13, č. 1 (2004), s. 89-100. ISSN 0962-1075. E-ISSN 1365-2583
Grant - others:NIH FIRCA(US) R03TWO1209-01; National Institute of Allergy and Infectious Diseases(US) AI46435; National Institute of Allergy and Infectious Diseases(US) AI46753
Institutional research plan: CEZ:AV0Z5007907
Keywords : Sindbis * alphavirus * mosquito
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.688, year: 2004
Following per os infection, a new Sindbis virus 5'dsMRE16ic-EGFP was detected in the gut of Aedes aegypti and Culex tritaeniorhynchus, but not Culex pipiens pipiens. Upon parenteral infection of larvae, the silkworm Bombyx mori showed EGFP expression in larvae, pupae and adults, while the tobacco hornworm Manduca sexta was resistant to the virus. 5'dsMRE16ic should be useful for gene expression in some important insect species that are otherwise difficult to manipulate genetically
Po infekci per os byl nový virus Sindbis 5'dsMRE16ic-EGFP detegován ve střevě komárů Aedes aegypti and Culex tritaeniorhynchus, ale ne Culex pipiens pipiens. Po parenterální infekci larev se exprese EGFP objevila v larvách, kuklách a dospělcích bource Bombyx mori, zatímco lišaj Manduca sexta byl vůči viru resistentní. 5'dsMRE16ic může být užitečný pro genovou expresi u některých důležitých druhů hmyzu, jejichž genetická manipulace by byla jinak obtížná
Permanent Link: http://hdl.handle.net/11104/0009028
Number of the records: 1