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The reverse tetracycline-controlled transactivator rtT A2s-S2 is toxic in mouse embryonic stem cells
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SYSNO ASEP 0101011 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title The reverse tetracycline-controlled transactivator rtT A2s-S2 is toxic in mouse embryonic stem cells Title Transaktivátor trT A2s-S2, kontrolovaný tetracyklinem je pro myší embryonální kmenové buňky toxický Author(s) Bryja, Vítězslav (BFU-R) RID, ORCID
Pachernik, J. (CZ)
Kubala, L. (CZ)
Hampl, Aleš (UEM-P)
Dvořák, Petr (UEM-P)Source Title Reproduction Nutrition Development - ISSN 0926-5287
Roč. 43, č. 6 (2003), s. 477-486Number of pages 10 s. Language eng - English Country FR - France Keywords tetracycline-controlled Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA204/01/0905 GA ČR - Czech Science Foundation (CSF) LN00A065 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z5039906 - UEM-P Annotation The efficient and reversible control of transgene expression is a powerful tool for correct manipulation of embryonic stem cells in both cell therapy and transgenesis. The aim of this work was to investigate the possibilities of recently developed reverse tetracycline controlled transactivator rtTA2-S2. It was shown that the rtTA2s-S2 is useful for transient inducible expression of genes stem ceels. However, it was not possible to establish mouse embryonic stem cell lines stably expressing this transactivator. Using the viral IRES sequence which ncouples the expression of rtTA2s-S2 and neomycin phosphotransferase, was found that embryonic stem cells expressing rtTA2s-S2 are not capable of growing in the presence of G418. These results indicate that this transactivator is toxic to ES cells and raise the need for the development of other strategies for stable and inducible expression of genes in ES cells Workplace Institute of Experimental Medicine Contact Arzuv Čaryjeva, arzuv.caryjeva@iem.cas.cz, Tel.: 241 062 218, 296 442 218 Year of Publishing 2005
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