Number of the records: 1
Isoflavonoids are present in Arabidopsis thaliana despite the absence of any homologue to known isoflavonoid synthases
- 1.
SYSNO ASEP 0082528 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Isoflavonoids are present in Arabidopsis thaliana despite the absence of any homologue to known isoflavonoid synthases Title Isoflavonoidy jsou přítomny v huseníčku thalově navzdory absenci blízkého homologu známých isoflkavonoid syntéz Author(s) Lapčík, O. (CZ)
Honys, David (UEB-Q) RID, ORCID
Koblovská, R. (CZ)
Macková, Z. (CZ)
Vítková, M. (CZ)
Klejdus, B. (CZ)Source Title Plant Physiology and Biochemistry. - : Elsevier - ISSN 0981-9428
Roč. 44, 2-3 (2006), s. 106-114Number of pages 9 s. Language eng - English Country FR - France Keywords Arabidopsis thaliana ; Brassicaceae ; HPLC-MS Subject RIV EB - Genetics ; Molecular Biology R&D Projects GA525/03/0352 GA ČR - Czech Science Foundation (CSF) KJB6038409 GA AV ČR - Academy of Sciences of the Czech Republic (AV ČR) CEZ AV0Z50380511 - UEB-Q (2005-2011) Annotation Extracts from Arabidopsis thaliana leaves and inflorescence stalks and from Lepidium sativa (Brassicaceac) seedlings were analysed by HPLC-MS-SIM and by five isoflavonoid-specific ELISA methods after the HPLC fractionation of samples, in order to determine presence of isoflavonoids. Individual ELISAs were specific for daidzein, genistein, biochanin A and for their derivatives substituted either at the 4'- or at the 7-positions. Both analytical approaches revealed homologous spectra of isoflavonoids in both plant species. As the ononin specific immunoassay was not available this compound was only detected by HPLC-MS. Formononetin and prunetin represented the main aglycones followed by biochanin A, daidzein and genistein; sissotrin was the most abundant isoflavonoid glycoside followed by ononin, daidzin and genistin. The content of individual compounds ranged from a few micrograms up to 2.2 mg kg(-1) (dry weight). Expression profiles of A. thaliana genes homologous to enzymes involved in isoflavonoid synthesis and metabolism were extracted from publicly available transcriptomic datasets for various tissues. Genes likely to be involved in important steps of the isoflavonoid metabolism in A. thaliana were identified. However, in accord with the previously published data, no homologue to isoflavone synthases known from the Fabaceae plants was found. These aryl migrating enzymes belong to the CYP93C family that is absent in A. thaliana. We conclude that another gene must be responsible for biosynthesis of the isoflavone skeleton in Brassicaceae. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2007
Number of the records: 1