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Dynamic studies of antibody-antigen interactions with an electrolyte-gated organic transistor
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SYSNO ASEP 0604893 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Dynamic studies of antibody-antigen interactions with an electrolyte-gated organic transistor Author(s) Urbina, P.A.M. (IT)
Paradisi, A. (IT)
Hasler, R. (AT)
Sensi, M. (IT)
Berto, M. (IT)
Saygin, G.D. (IT)
Dostálek, Jakub (FZU-D) ORCID, RID
Pinti, M. (IT)
Greco, P. (IT)
Borsari, M. (IT)
Knoll, W. (AT)
Bortolotti, C.A. (IT)
Biscarini, F. (IT)Number of authors 13 Article number 101919 Source Title Cell Reports Physical Science. - : Cell Press
Roč. 5, č. 8 (2024)Number of pages 18 s. Language eng - English Country US - United States Keywords surface plasmon resonance ; field-effect transistor ; biosensor ; biomolecular interaction analysis Subject RIV BH - Optics, Masers, Lasers OECD category Optics (including laser optics and quantum optics) R&D Projects GC22-30456J GA ČR - Czech Science Foundation (CSF) Method of publishing Open access Institutional support FZU-D - RVO:68378271 UT WOS 001301222400001 EID SCOPUS 85191892001 DOI https://doi.org/10.1016/j.xcrp.2024.101919 Annotation Affinity-based biosensors employing surface-bound biomolecules for analyte detection are important tools in clinical diagnostics and drug development. In this context, electrolyte-gated organic transistors (EGOTs) are emerging as ultrasensitive label-free biosensors. In this study, we present an EGOT sensor integrated within a microfluidic system. The sensor utilizes the cytomegalovirus (CMV) phosphoprotein 65 as a biorecognition element to detect the pathological biomarker human anti-cytomegalovirus antibody in solution. The biorecognition element is grafted onto the gate electrode by exploiting the polyhistidine-tag technology. Real-time monitoring of the EGOT response, coupled with a two compartment kinetic model analysis, enables the determination of analyte concentration, binding kinetics, and thermodynamics of the interaction.
Workplace Institute of Physics Contact Kristina Potocká, potocka@fzu.cz, Tel.: 220 318 579 Year of Publishing 2025 Electronic address https://hdl.handle.net/11104/0362438
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