0604893 - FZÚ 2025 RIV US eng J - Journal Article
Urbina, P.A.M. - Paradisi, A. - Hasler, R. - Sensi, M. - Berto, M. - Saygin, G.D. - Dostálek, Jakub - Pinti, M. - Greco, P. - Borsari, M. - Knoll, W. - Bortolotti, C.A. - Biscarini, F.
Dynamic studies of antibody-antigen interactions with an electrolyte-gated organic transistor.
Cell Reports Physical Science. Roč. 5, č. 8 (2024), č. článku 101919. E-ISSN 2666-3864
R&D Projects: GA ČR(CZ) GC22-30456J
Institutional support: RVO:68378271
Keywords : surface plasmon resonance * field-effect transistor * biosensor * biomolecular interaction analysis
OECD category: Optics (including laser optics and quantum optics)
Impact factor: 7.9, year: 2023 ; AIS: 2.092, rok: 2023
Method of publishing: Open access
DOI: https://doi.org/10.1016/j.xcrp.2024.101919

Affinity-based biosensors employing surface-bound biomolecules for analyte detection are important tools in clinical diagnostics and drug development. In this context, electrolyte-gated organic transistors (EGOTs) are emerging as ultrasensitive label-free biosensors. In this study, we present an EGOT sensor integrated within a microfluidic system. The sensor utilizes the cytomegalovirus (CMV) phosphoprotein 65 as a biorecognition element to detect the pathological biomarker human anti-cytomegalovirus antibody in solution. The biorecognition element is grafted onto the gate electrode by exploiting the polyhistidine-tag technology. Real-time monitoring of the EGOT response, coupled with a two compartment kinetic model analysis, enables the determination of analyte concentration, binding kinetics, and thermodynamics of the interaction.

Permanent Link: https://hdl.handle.net/11104/0362438