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Direct Analysis of Dried Blood Spots by Automated Sequential Injection-Capillary Electrophoresis
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SYSNO ASEP 0552251 Document Type A - Abstract R&D Document Type The record was not marked in the RIV R&D Document Type Není vybrán druh dokumentu Title Direct Analysis of Dried Blood Spots by Automated Sequential Injection-Capillary Electrophoresis Author(s) Dvořák, Miloš (UIACH-O)
Ryšavá, Lenka (UIACH-O) RID, ORCID, SAI
Miró, M. (ES)
Kubáň, Pavel (UIACH-O) RID, ORCIDNumber of authors 4 Source Title Virtual Lace 2021. Book of Abstracts. -, 2021 / Guzman N. A. ; da Silva J. A. F.
S. 62-63Number of pages 2 s. Publication form Online - E Action VIRTUAL LACE 2021. Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology /26./ Event date 04.12.2021 - 07.12.2021 VEvent location Virtual Edition Country BR - Brazil Event type WRD Language eng - English Country BR - Brazil Keywords dried blood spots ; capillary electrophoresis Subject RIV CB - Analytical Chemistry, Separation OECD category Analytical chemistry R&D Projects GA18-13135S GA ČR - Czech Science Foundation (CSF) Institutional support UIACH-O - RVO:68081715 Annotation In our laboratories, automated liquid handling by sequential injection (SI) has been developed as a front end to modern analytical instrumentation and coupled to capillary electrophoresis (CE). In order to resort to the favourable synergetic aspects of SI and CE and to complement/broaden the portfolio of automated DBS analytical set-ups, the actual contribution presents a novel proof-of-concept study for a fully automated DBS analysis platform. To this end, a complete DBS is autonomously eluted with an SI system (thus free from haematocrit/non-homogeneity effects), which is at-line coupled to the internal port of a CE autosampler. The two commercial instruments are interconnected through standard tubing, ferrules, and nuts, require no additional instrumental adjustments, are operated by dedicated software, and are synchronized for full automation. The SI-CE coupling offers a reliable and unattended handling of minute reagents and sample volumes, rapid analysis, and sufficient sensitivity for the determination of endogenous and exogenous compounds in DBSs. The proposed concept reflects the actual analytical trends in automation and provides a general solution to modern clinical analysis as it can be applied to a broad range of analytes and dried biological materials. Workplace Institute of Analytical Chemistry Contact Iveta Drobníková, drobnikova@iach.cz, Tel.: 532 290 234 Year of Publishing 2023
Number of the records: 1