Direct Analysis of Dried Blood Spots by Automated Sequential Injection-Capillary Electrophoresis

0552251 - ÚIACH 2023 BR eng A - Abstract
Dvořák, Miloš - Ryšavá, Lenka - Miró, M. - Kubáň, Pavel
Direct Analysis of Dried Blood Spots by Automated Sequential Injection-Capillary Electrophoresis.
Virtual Lace 2021. Book of Abstracts. 2021 - (Guzman, N.; da Silva, J.). s. 62-63
[VIRTUAL LACE 2021. Latin-American Symposium on Biotechnology, Biomedical, Biopharmaceutical, and Industrial Applications of Capillary Electrophoresis and Microchip Technology /26./. 04.12.2021-07.12.2021, Virtual Edition]
R&D Projects: GA ČR(CZ) GA18-13135S
Institutional support: RVO:68081715
Keywords : dried blood spots * capillary electrophoresis
OECD category: Analytical chemistry

In our laboratories, automated liquid handling by sequential injection (SI) has been developed as a front end to modern analytical instrumentation and coupled to capillary electrophoresis (CE). In order to resort to the favourable synergetic aspects of SI and CE and to complement/broaden the portfolio of automated DBS analytical set-ups, the actual contribution presents a novel proof-of-concept study for a fully automated DBS analysis platform. To this end, a complete DBS is autonomously eluted with an SI system (thus free from haematocrit/non-homogeneity effects), which is at-line coupled to the internal port of a CE autosampler. The two commercial instruments are interconnected through standard tubing, ferrules, and nuts, require no additional instrumental adjustments, are operated by dedicated software, and are synchronized for full automation. The SI-CE coupling offers a reliable and unattended handling of minute reagents and sample volumes, rapid analysis, and sufficient sensitivity for the determination of endogenous and exogenous compounds in DBSs. The proposed concept reflects the actual analytical trends in automation and provides a general solution to modern clinical analysis as it can be applied to a broad range of analytes and dried biological materials.
Permanent Link: http://hdl.handle.net/11104/0327378