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Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase
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SYSNO ASEP 0494802 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase Author(s) Proctor, M.S. (GB)
Chidgey, J.W. (GB)
Shukla, Mahendra K. (MBU-M) RID
Jackson, P. J. (GB)
Sobotka, Roman (MBU-M) RID, ORCID
Hunter, C.N. (GB)
Hitchcock, A. (GB)Source Title FEBS Letters. - : Wiley - ISSN 0014-5793
Roč. 592, č. 18 (2018), s. 3062-3073Number of pages 12 s. Language eng - English Country US - United States Keywords Arabidopsis ; chlorophyll synthase ; cyanobacteria Subject RIV CE - Biochemistry OECD category Biochemistry and molecular biology R&D Projects LO1416 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) GA17-08755S GA ČR - Czech Science Foundation (CSF) Institutional support MBU-M - RVO:61388971 UT WOS 000445330800005 EID SCOPUS 85053276252 DOI 10.1002/1873-3468.13222 Annotation In the model cyanobacterium Synechocystis sp. PCC 6803, the terminal enzyme of chlorophyll biosynthesis, chlorophyll synthase (ChlG), forms a complex with high light-inducible proteins, the photosystem II assembly factor Ycf39 and the YidC/Alb3/Oxal membrane insertase, co-ordinating chlorophyll delivery with cotranslational insertion of nascent photosystem polypeptides into the membrane. To gain insight into the ubiquity of this assembly complex in higher photosynthetic organisms, we produced functional foreign chlorophyll syntheses in a cyanobacterial host. Synthesis of algal and plant chlorophyll syntheses allowed deletion of the otherwise essential native cyanobacterial gene. Analysis of purified protein complexes shows that the interaction with YidC is maintained for both eukaryotic enzymes, indicating that a ChlG-YidC/Alb3 complex may be evolutionarily conserved in algae and plants. Workplace Institute of Microbiology Contact Eliška Spurná, eliska.spurna@biomed.cas.cz, Tel.: 241 062 231 Year of Publishing 2019
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