Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase

0494802 - MBÚ 2019 RIV US eng J - Journal Article
Proctor, M.S. - Chidgey, J.W. - Shukla, Mahendra K. - Jackson, P. J. - Sobotka, Roman - Hunter, C.N. - Hitchcock, A.
Plant and algal chlorophyll synthases function in Synechocystis and interact with the YidC/Alb3 membrane insertase.
FEBS Letters. Roč. 592, č. 18 (2018), s. 3062-3073. ISSN 0014-5793. E-ISSN 1873-3468
R&D Projects: GA MŠMT(CZ) LO1416; GA ČR(CZ) GA17-08755S
Institutional support: RVO:61388971
Keywords : Arabidopsis * chlorophyll synthase * cyanobacteria
OECD category: Biochemistry and molecular biology
Impact factor: 2.675, year: 2018

In the model cyanobacterium Synechocystis sp. PCC 6803, the terminal enzyme of chlorophyll biosynthesis, chlorophyll synthase (ChlG), forms a complex with high light-inducible proteins, the photosystem II assembly factor Ycf39 and the YidC/Alb3/Oxal membrane insertase, co-ordinating chlorophyll delivery with cotranslational insertion of nascent photosystem polypeptides into the membrane. To gain insight into the ubiquity of this assembly complex in higher photosynthetic organisms, we produced functional foreign chlorophyll syntheses in a cyanobacterial host. Synthesis of algal and plant chlorophyll syntheses allowed deletion of the otherwise essential native cyanobacterial gene. Analysis of purified protein complexes shows that the interaction with YidC is maintained for both eukaryotic enzymes, indicating that a ChlG-YidC/Alb3 complex may be evolutionarily conserved in algae and plants.
Permanent Link: http://hdl.handle.net/11104/0287872