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Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples
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SYSNO ASEP 0373461 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Článek ve WOS Title Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples Author(s) Campuzano, S. (US)
Kuralay, F. (US)
Lobo-Castanón, M.J. (US)
Bartošík, Martin (BFU-R) RID
Vyavahare, K. (US)
Paleček, Emil (BFU-R) RID, ORCID
Haake, D.A. (US)
Wang, J. (US)Number of authors 8 Source Title Biosensors and Bioelectronics. - : Elsevier - ISSN 0956-5663
Roč. 26, č. 8 (2011), s. 3577-3583Number of pages 7 s. Language eng - English Country NL - Netherlands Keywords electrochemical detection ; DNA hybridization ; self-assembled monolayer Subject RIV BO - Biophysics R&D Projects ME09038 GA MŠMT - Ministry of Education, Youth and Sports (MEYS) CEZ AV0Z50040507 - BFU-R (2005-2011) AV0Z50040702 - BFU-R (2007-2013) UT WOS 000289863900030 DOI 10.1016/j.bios.2011.02.004 Annotation We present a highly sensitive electrochemical genosensor for direct measurement of specific DNA sequences in undiluted and untreated human serum and urine samples. The method relies on a new ternary interface involving hexanedithiol co-immobilized with the thiolated capture probe on gold surfaces, followed by the incorporation of 6-mercapto-1-hexanol as diluent. The new ternary layer led to a 80-fold improvement in the signal-to-noise ratio for 1 nM target DNA in undiluted human serum over the common binary alkanethiol interface, and allowed the direct quantification of the target DNA down to 7 pM (28 amol) and 17pM (68 amol) in untreated serum and urine, respectively. Workplace Institute of Biophysics Contact Jana Poláková, polakova@ibp.cz, Tel.: 541 517 244 Year of Publishing 2012
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