Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples

0373461 - BFÚ 2012 RIV NL eng J - Journal Article
Campuzano, S. - Kuralay, F. - Lobo-Castanón, M.J. - Bartošík, Martin - Vyavahare, K. - Paleček, Emil - Haake, D.A. - Wang, J.
Ternary monolayers as DNA recognition interfaces for direct and sensitive electrochemical detection in untreated clinical samples.
Biosensors and Bioelectronics. Roč. 26, č. 8 (2011), s. 3577-3583. ISSN 0956-5663. E-ISSN 1873-4235
R&D Projects: GA MŠMT(CZ) ME09038
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : electrochemical detection * DNA hybridization * self-assembled monolayer
Subject RIV: BO - Biophysics
Impact factor: 5.602, year: 2011

We present a highly sensitive electrochemical genosensor for direct measurement of specific DNA sequences in undiluted and untreated human serum and urine samples. The method relies on a new ternary interface involving hexanedithiol co-immobilized with the thiolated capture probe on gold surfaces, followed by the incorporation of 6-mercapto-1-hexanol as diluent. The new ternary layer led to a 80-fold improvement in the signal-to-noise ratio for 1 nM target DNA in undiluted human serum over the common binary alkanethiol interface, and allowed the direct quantification of the target DNA down to 7 pM (28 amol) and 17pM (68 amol) in untreated serum and urine, respectively.
Permanent Link: http://hdl.handle.net/11104/0206555