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Induction of RNA-mediated resistance to papaya ringspot virus type W
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SYSNO ASEP 0305977 Document Type J - Journal Article R&D Document Type Journal Article Subsidiary J Ostatní články Title Induction of RNA-mediated resistance to papaya ringspot virus type W Title Indukce rezistence řízené RNA k viru kroužkovitosti papáje typu W Author(s) Krubphachaya, P. (TH)
Juříček, Miloslav (UEB-Q) RID, ORCID
Kertbundit, Sunee (UEB-Q)Source Title Journal of Biochemistry and Molecular Biology - ISSN 1225-8687
Roč. 40, č. 3 (2007), s. 404-411Number of pages 8 s. Language eng - English Country SG - Singapore Keywords inverted-repeat ; in vitro inoculation ; PRSV type W Subject RIV EB - Genetics ; Molecular Biology Next source Other public resources CEZ AV0Z50380511 - UEB-Q (2005-2011) Annotation Transformation of cantaloupes with the coat protein (cp) gene of papaya ringspot virus type W (PRSV-W), Thai isolate, was used to introduce virus resistance. Binary vectors containing either the full length coat protein coding region under control of the 35S CaMV promoter (pSA1175), or the inverted-repeat of a coat protein coding region (pSA1304), were constructed and used for Agrobacterium-mediated transformation of cotyledonary explants of the cantaloupe cultivar Sun Lady. Four independent transgenic tines were obtained using pSA1304 and one using pSA1175. Integration of the PRSV-W cp gene into the genome of these transgenic lines was verified by PCR amplification, GUS assays and Southern blot hybridization. In vitro inoculation of these tines with PRSV-W revealed that whereas the fine containing pSA1175 remained sensitive, the four lines containing pSA1304 were resistant. The presence of small RNA species, presumably siRNA, corresponding to regions of the viral cp gene in transgenic tines resistant to PRSV-W supports the involvement of post-transcriptional gene silencing in the establishment of resistance. Workplace Institute of Experimental Botany Contact David Klier, knihovna@ueb.cas.cz, Tel.: 220 390 469 Year of Publishing 2008
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