0305977 - ÚEB 2008 RIV SG eng J - Journal Article
Krubphachaya, P. - Juříček, Miloslav - Kertbundit, Sunee
Induction of RNA-mediated resistance to papaya ringspot virus type W.
[Indukce rezistence řízené RNA k viru kroužkovitosti papáje typu W.]
Journal of Biochemistry and Molecular Biology. Roč. 40, č. 3 (2007), s. 404-411. ISSN 1225-8687
Grant - others:BIOTEC, NSTDA(TH) BT-B-06-PG-14-4503
Institutional research plan: CEZ:AV0Z50380511
Source of funding: V - Other public resources
Keywords : inverted-repeat * in vitro inoculation * PRSV type W
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 2.141, year: 2007 ; AIS: 0.532, rok: 2007
Result website:
http://www.jbmb.or.kr/view_article.php3?cont=jbmb&kid=182&mid=13&pid=13

Transformation of cantaloupes with the coat protein (cp) gene of papaya ringspot virus type W (PRSV-W), Thai isolate, was used to introduce virus resistance. Binary vectors containing either the full length coat protein coding region under control of the 35S CaMV promoter (pSA1175), or the inverted-repeat of a coat protein coding region (pSA1304), were constructed and used for Agrobacterium-mediated transformation of cotyledonary explants of the cantaloupe cultivar Sun Lady. Four independent transgenic tines were obtained using pSA1304 and one using pSA1175. Integration of the PRSV-W cp gene into the genome of these transgenic lines was verified by PCR amplification, GUS assays and Southern blot hybridization. In vitro inoculation of these tines with PRSV-W revealed that whereas the fine containing pSA1175 remained sensitive, the four lines containing pSA1304 were resistant. The presence of small RNA species, presumably siRNA, corresponding to regions of the viral cp gene in transgenic tines resistant to PRSV-W supports the involvement of post-transcriptional gene silencing in the establishment of resistance.

Agrotransformace Cucumis melo binárním vektorem nesoucím inverzní repetitivní sekvenci genu pro obalový protein viru kroužovitosti papáje typu W byla použita pro cílené navození rezistence C. melo k tomuto viru. Byly získány čtyři rezistentní linie, které akumulovaly siRNA a tedy rezistence byla řízena RNA na bázi PTGS.
Permanent Link: http://hdl.handle.net/11104/0159098