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Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo

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    0350885 - ÚPT 2011 RIV CH eng J - Journal Article
    Samek, Ota - Jonáš, Alexandr - Pilát, Zdeněk - Zemánek, Pavel - Nedbal, Ladislav - Tříska, Jan - Kotas, Petr - Trtílek, M.
    Raman Microspectroscopy of Individual Algal Cells: Sensing Unsaturation of Storage Lipids in vivo.
    Sensors. Roč. 10, č. 9 (2010), s. 8635-8651. ISSN 1424-8220
    R&D Projects: GA MŠk ED0017/01/01; GA MŠk OC08034; GA MPO FR-TI1/433; GA ČR GA206/09/1284
    Grant - others:EU(XE) PERG 06-GA-2009-256526
    Institutional research plan: CEZ:AV0Z20650511; CEZ:AV0Z60870520
    Keywords : Raman spectroscopy * algal cells * lipids * iodine value
    Subject RIV: JA - Electronics ; Optoelectronics, Electrical Engineering
    Impact factor: 1.771, year: 2010

    lgae are becoming a strategic source of fuels, food, feedstocks, and biologically active compounds. This potential has stimulated the development of innovative analytical methods focused on these microorganisms. Algal lipids are among the most promising potential products for fuels as well as for nutrition. The crucial parameter characterizing the algal lipids is the degree of unsaturation of the constituent fatty acids quantified by the iodine value. Here we demonstrate the capacity of the spatially resolved Raman microspectroscopy to determine the effective iodine value in lipid storage bodies of individual living algal cells. The Raman spectra were collected from three selected algal species immobilized in an agarose gel.
    Permanent Link: http://hdl.handle.net/11104/0190765
     
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