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Synthesis of photon-upconversion nanoparticles for microfluidic single-molecule assays
- 1.0576910 - ÚIACH 2024 CZ eng A - Abstract
Weisová, Julie - Hlaváček, Antonín - Křivánková, Jana
Synthesis of photon-upconversion nanoparticles for microfluidic single-molecule assays.
12th International Conference Analytical Cytometry : Book of abstracts. Praha: AMCA, spol. s. r. o., 2023. s. 1-1.
[International Conference Analytical Cytometry /12./. 02.09.2023-05.09.2023, Praha]
R&D Projects: GA ČR(CZ) GA21-03156S
Institutional support: RVO:68081715
Keywords : photon-upconversion nanoparticles * surface modification
OECD category: Analytical chemistry
https://www.conference.csac.cz/Amca-CSAC/media/content/2023/docs/Book_of_abstracts_CSAC_2023.pdf
Photon-upconversion nanoparticles (UCNPs) are luminescent nanocrystals, which are doped with lanthanide ions. UCNPs are excited by near-infrared light (976 nm) and emit light of shorter wavelengths in both visible and near-infrared ranges. Photon-upconversion luminescence is detected without a background autofluorescence, which makes UCNPs extremely suitable for single- molecule assays. Here, we describe the development and optimization of UCNPs for microfluidic single-molecule bioaffinity assays. The size, composition, and internal architecture of UCNPs are carefully tuned to provide bright emission. The core-shell structure was optimal for Er3+ doped nanoparticles (emission wavelengths 554 nm and 660 nm), and the core-only structure was used in Tm3+ doped nanoparticles (emission wavelengths 450 nm and 802 nm). Two types of surface modification were tested. The first type utilized silanization, and the second one encapsulated the UCNPs into a hydrophilic acrylic polymer. The monodispersity of all samples is characterized by optical microscopy and single-nanoparticle counting is used for absolute quantification.
Permanent Link: https://hdl.handle.net/11104/0346296
Number of the records: 1