DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time

0566160 - FZÚ 2023 RIV GB eng J - Journal Article
Keta, O. - Petkovic, V. - Cirrone, P. - Petringa, Giada - Cuttone, G. - Sakata, D. - Shin, W-G. - Incerti, S. - Petrovic, I. - Fira, A.R.
DNA double-strand breaks in cancer cells as a function of proton linear energy transfer and its variation in time.
International Journal of Radiation Biology. Roč. 97, č. 9 (2021), s. 1229-1240. ISSN 0955-3002. E-ISSN 1362-3095
Institutional support: RVO:68378271
Keywords : human malignant cells * gamma-rays * protons * carbon ions * DNA double-strand breaks
OECD category: Particles and field physics
Impact factor: 3.352, year: 2021
Method of publishing: Limited access

Purpose The complex relationship between linear energy transfer (LET) and cellular response to radiation is not yet fully elucidated. To better characterize DNA damage after irradiations with therapeutic protons, we monitored formation and disappearance of DNA double-strand breaks (DNA DSB) as a function of LET and time. Comparisons with conventional gamma-rays and high LET carbon ions were also performed. Materials and Methods In the present work, we performed immunofluorescence-based assay to determine the amount of DNA DSB induced by different LET values along the 62 MeV therapeutic proton Spread out Bragg peak (SOBP) in three cancer cell lines, i.e. HTB140 melanoma, MCF-7 breast adenocarcinoma and HTB177 non-small lung cancer cells. Time dependence of foci formation was followed as well. To determine irradiation positions, corresponding to the desired LET values, numerical simulations were carried out using Geant4 toolkit.
Permanent Link: https://hdl.handle.net/11104/0337588