High Cysteine Diet Reduces Insulin Resistance in SHR-CRP Rats

0550770 - FGÚ 2022 RIV CZ eng J - Journal Article
Krijt, J. - Sokolová, J. - Šilhavý, Jan - Mlejnek, Petr - Kubovčiak, Jan - Liška, František - Malínská, H. - Hüttl, M. - Marková, I. - Křížková, M. - Stipanuk, M. H. - Křížek, T. - Ditroi, T. - Nagy, P. - Kožich, V. - Pravenec, Michal
High Cysteine Diet Reduces Insulin Resistance in SHR-CRP Rats.
Physiological Research. Roč. 70, č. 5 (2021), s. 687-700. ISSN 0862-8408. E-ISSN 1802-9973
R&D Projects: GA MZd(CZ) NV16-30384A; GA MŠMT(CZ) LM2018131
Research Infrastructure: ELIXIR-CZ II - 90131
Institutional support: RVO:67985823 ; RVO:68378050
Keywords : SHR-CRP rats * metabolic syndrome * taurine * cysteine * insulin resistance * cysteine dioxygenase
OECD category: Endocrinology and metabolism (including diabetes, hormones)
Impact factor: 2.139, year: 2021
Method of publishing: Open access
https://www.biomed.cas.cz/physiolres/pdf/2021/70_687.pdf

Increased plasma total cysteine (tCys) has been associated with obesity and metabolic syndrome in human and some animal studies but the underlying mechanisms remain unclear. In this study, we aimed at evaluating the effects of high cysteine diet administered to SHR-CRP transgenic rats, a model of metabolic syndrome and inflammation. SHR-CRP rats were fed either standard (3.2 g cystine/kg diet) or high cysteine diet (HCD, enriched with additional 4 g L-cysteine/kg diet). After 4 weeks, urine, plasma and tissue samples were collected and parameters of metabolic syndrome, sulfur metabolites and hepatic gene expression were evaluated. Rats on HCD exhibited similar body weights and weights of fat depots, reduced levels of serum insulin, and reduced oxidative stress in the liver. The HCD did not change concentrations of tCys in tissues and body fluids while taurine in tissues and body fluids, and urinary sulfate were significantly increased. In contrast, betaine levels were significantly reduced possibly compensating for taurine elevation. In summary, increased Cys intake did not induce obesity while it ameliorated insulin resistance in the SHR-CRP rats, possibly due to beneficial effects of accumulating taurine.
Permanent Link: http://hdl.handle.net/11104/0326086