High-performance countercurrent chromatography for lutein production from a chlorophyll-deficient strain of the microalgae Parachlorella kessleri HY1

0544824 - MBÚ 2022 RIV US eng J - Journal Article
Fábryová, Tereza - Kubáč, David - Kuzma, Marek - Hrouzek, Pavel - Kopecký, Jiří - Tůmová, L. - Cheel Horna, José
High-performance countercurrent chromatography for lutein production from a chlorophyll-deficient strain of the microalgae Parachlorella kessleri HY1.
Journal of Applied Phycology. Roč. 33, č. 4 (2021), s. 1999-2013. ISSN 0921-8971. E-ISSN 1573-5176
R&D Projects: GA MPO FV10155; GA MŠMT(CZ) LO1416
Institutional support: RVO:61388971
Keywords : Lutein * Microalgae Parachlorella kessleri * High-performance countercurrent chromatography (HPCCC) * Liquid * liquid chromatography
OECD category: Microbiology
Impact factor: 3.404, year: 2021
Method of publishing: Limited access
https://link.springer.com/article/10.1007%2Fs10811-021-02434-y

Green microalgae are a recognized lutein source, however, processing for lutein production requires additional operations such as extraction and saponification, mainly due to the high green pigment and lipid content in the biomass. In this study lutein was isolated from a chlorophyll-deficient Parachlorella kessleri HY1 strain using high-performance countercurrent chromatography (HPCCC). The lower phase of the biphasic solvent system composed of n-heptane-ethanol-water, 5:4:1.5, v/v/v was used both as biomass extraction solvent and HPCCC mobile phase conferring a high selectivity to the lutein production process. For the HPCCC isolation, a multiple injection method was developed, and ten consecutive sample injections (300 mg per each) were performed. To favor the economics of the process, the HPCCC mobile and stationary phases were separately formulated based on nuclear magnetic resonance (NMR) analyses. This strategy enabled to avoid obtaining immiscible liquid phases from their parent biphasic solvent system, which led to the reduction of the separation process duration and solvent consumption. Overall, 3 g of P. kessleri HY1 strain extract was processed by HPCCC yielding 150 mg of lutein (95% purity, 97% recovery). The results presented here form an efficient and economical basis for the large-scale production of microalgae-sourced lutein.
Permanent Link: http://hdl.handle.net/11104/0321636