Laccase and horseradish peroxidase for green treatment of phenolic micropollutants in real drinking water and wastewater

0544749 - MBÚ 2022 RIV DE eng J - Journal Article
Marysková, M. - Linhartová, Lucie - Novotný, V. - Rysová, M. - Cajthaml, Tomáš - Ševců, A.
Laccase and horseradish peroxidase for green treatment of phenolic micropollutants in real drinking water and wastewater.
Environmental Science and Pollution Research. Roč. 28, č. 24 (2021), s. 31566-31574. ISSN 0944-1344. E-ISSN 1614-7499
R&D Projects: GA TA ČR TE01020218
Institutional support: RVO:61388971
Keywords : Anthropogenic pollution * Biodegradation * Enzymatic treatment * Endocrine-disrupting chemicals * Ecotoxicity * Wastewater treatment
OECD category: Environmental biotechnology
Impact factor: 5.190, year: 2021
Method of publishing: Limited access
https://link.springer.com/article/10.1007%2Fs11356-021-12910-0

Biologically active micropollutants that contain diverse phenolic/aromatic structures are regularly present in wastewater effluents and are even found in drinking water. Advanced green technologies utilizing immobilized laccase and/or peroxidase, which target these micropollutants directly, may provide a reasonable alternative to standard treatments. Nevertheless, the use of these enzymes is associated with several issues that may prevent their application, such as the low activity of laccase at neutral and basic pH or the necessity of hydrogen peroxide addition as a co-substrate for peroxidases. In this study, the activity of laccase from Trametes versicolor and horseradish peroxidase was evaluated across a range of commonly used substrates (2,2 '-azino-bis(3-ethylbenzothiazoline-6-sulphonic acid) (ABTS), syringaldazine, and guaiacol). Moreover, conditions for their optimal performance were explored along with an assessment of whether these conditions accurately reflect the effectivity of both enzymes in the degradation of a mixture of bisphenol A, 17 alpha-ethinylestradiol, triclosan, and diclofenac in tap drinking water and secondary wastewater effluent. Laccase and horseradish peroxidase showed optimal activity at strongly acidic pH if ABTS was used as a substrate. Correspondingly, the activities of both enzymes detected using ABTS in real waters were significantly enhanced by adding approximately 2.5% (v/v) of McIlvaine's buffer. Degradation of a mixture of micropollutants in wastewater with 2.5% McIlvaine's buffer (pH 7) resulted in a substantial decrease in estrogenic activity. Low degradation efficiency of micropollutants by laccase was observed in pure McIlvaine's buffer of pH 3 and 7, compared with efficient degradation in tap water of pH 7.5 without buffer.
Permanent Link: http://hdl.handle.net/11104/0321576