The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote

0543232 - MBÚ 2022 RIV NL eng J - Journal Article
Vítová, Milada - Lanta, Vojtěch - Čížková, Mária - Jakubec, M. - Rise, F. - Halskau, O. - Bišová, Kateřina - Furse, S.
The biosynthesis of phospholipids is linked to the cell cycle in a model eukaryote.
Biochimica Et Biophysica Acta-Molecular and Cell Biology of Lipids. Roč. 1866, č. 8 (2021), č. článku 158965. ISSN 1388-1981. E-ISSN 1879-2618
EU Projects: European Commission(CZ) ATCZ172 - Interreg V-A Austria – Czech Republic
Institutional support: RVO:61388971
Keywords : phase-transitions * chlamydomonas-reinhardtii * dependent kinases * cleavage furrow * green-algae * yeast * lipids * proteins * phosphatidylethanolamine * redistribution * Cell cycle * Cell division * Cell structure * Desmodesmus quadricauda * Green algae * Lipid composition * Lipid metabolism
OECD category: Microbiology
Impact factor: 5.228, year: 2021
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S1388198121000937?via%3Dihub

The structural challenges faced by eukaryotic cells through the cell cycle are key for understanding cell viability and proliferation. We tested the hypothesis that the biosynthesis of structural lipids is linked to the cell cycle. If true, this would suggest that the cell's structure is important for progress through and perhaps even control of the cell cycle. Lipidomics (P-31 NMR and MS), proteomics (Western immunoblotting) and transcriptomics (RT-qPCR) techniques were used to profile the lipid fraction and characterise aspects of its metabolism at seven stages of the cell cycle of the model eukaryote, Desmodesmus quadricauda. We found considerable, transient increases in the abundance of phosphatidylethanolamine during the G(1) phase (+35%, ethanolamine phosphate cytidylyltransferase increased 2.5x) and phosphatidylglycerol (+100%, phosphatidylglycerol synthase increased 22x) over the G1/pre-replication phase boundary. The relative abundance of phosphatidylcholine fell by similar to 35% during the G1. N-Methyl transferases for the conversion of phosphatidylethanolamine into phosphatidylcholine were not found in the de novo transcriptome profile, though a choline phosphate transferase was found, suggesting that the Kennedy pathway is the principal route for the synthesis of PC. The fatty acid profiles of the four most abundant lipids suggested that these lipids were not generally converted between one another. This study shows for the first time that there are considerable changes in the biosynthesis of the three most abundant phospholipid classes in the normal cell cycle of D. quadricauda, by margins large enough to elicit changes to the physical properties of membranes.
Permanent Link: http://hdl.handle.net/11104/0320486