Kupffer Cells: Methods and Protocols

0534399 - FGÚ 2021 RIV US eng M - Monography Chapter
Tencerová, Michaela
Glucan-Encapsulated siRNA Particles (GeRPs) for Specific Gene Silencing in Kupffer Cells in Mouse Liver.
Kupffer Cells: Methods and Protocols. New York: Humana Press, 2020 - (Aouadi, M.; Azzimato, V.), s. 65-73. Methods in Molecular Biology, 2164. ISBN 978-1-0716-0704-6
R&D Projects: GA ČR(CZ) GA20-03586S
Institutional support: RVO:67985823
Keywords : RNAi delivery * intravenous administration * Kupffer cells * mouse liver * gene silencing * obesity * diabetes * inflammation * insulin sensitivity
OECD category: Cell biology
https://link.springer.com/protocol/10.1007%2F978-1-0716-0704-6_8

Here, we describe a protocol to prepare and administer glucan-encapsulated RNAi particles (GeRPs), for specific delivery of siRNA and subsequent gene silencing in Kupffer cells (KCs) in mice. This technology is based on baker’s yeast and allows gene manipulation in macrophages in a tissue-specific manner depending on the route of administration and the model that is used. GeRP administered by intravenous injection in mice are delivered to KCs. Therefore, using the GeRP technology to silence genes provides a unique method to study the function of factors expressed by KCs in the regulation of liver function.
Permanent Link: http://hdl.handle.net/11104/0312601