Luminescent Nanocomposites for Biomedical Applications

0531039 - ÚIACH 2021 RIV US eng J - Journal Article
Popovich, K. - Klepárník, Karel - Ledvina, Vojtěch - Neužilová, B. - Fleišmann, J. - Škodová, M. - Kobera, Libor - Mihóková, Eva - Múčka, V. - Čuba, V.
Luminescent Nanocomposites for Biomedical Applications.
IEEE Transactions on Nuclear Science. Roč. 67, č. 6 (2020), s. 962-968, č. článku 9000703. ISSN 0018-9499. E-ISSN 1558-1578
R&D Projects: GA ČR(CZ) GA17-01995S; GA ČR(CZ) GA20-06374S
Institutional support: RVO:68081715 ; RVO:68378271 ; RVO:61389013
Keywords : nanocomposite * nanoparticles * cathodoluminescence * scanning electron microscopy
OECD category: Analytical chemistry; Polymer science (UMCH-V); Nano-materials (production and properties) (FZU-D)
Impact factor: 1.679, year: 2020
Method of publishing: Limited access

In our work, we pursue a concept of the multimodal drug preparation based on LuAG:Ce3+ nanoparticles. The functionality studied in this paper is focused on the bioimaging of living cells via cathodoluminescence of scintillating nanoparticles attached to cell surface. LuAG:Ce3+ scintillating nanoparticles were used as a core, amorphous or mesoporous SiO2 was used as a shell encapsulating the core to increase the surface reactivity, anti-FasL antibody was then attached to the outer shell in order to specifically bond the nanocomposite to the cell receptors, finally, polyethylene glycol molecules were attached to increase the biocompatibility of the nanocomposites. Cathodoluminescence bioimaging was tested on yeasts Saccharomyces cerevisiae and leukocytes. It was found that the nanocomposites are able to attach to the cells via sorption or bonding to the receptors. Prepared nanocomposite shows stable luminescence under 10 keV electron irradiation, thus making a prospective candidate for this application and future multimodal drug design.
Permanent Link: http://hdl.handle.net/11104/0309801