Retention mechanism of branched macromolecules in size exclusion chromatography

0525298 - ÚMCH 2021 RIV US eng J - Journal Article
Netopilík, Miloš - Podzimek, Š.
Retention mechanism of branched macromolecules in size exclusion chromatography.
ACS Omega. Roč. 5, č. 24 (2020), s. 14254-14260. ISSN 2470-1343. E-ISSN 2470-1343
R&D Projects: GA ČR(CZ) GC17-04258J
Institutional support: RVO:61389013
Keywords : size-exclusion chromatography * random branching * separation mechanism
OECD category: Polymer science
Impact factor: 3.512, year: 2020
Method of publishing: Open access
https://pubs.acs.org/doi/10.1021/acsomega.9b03952#

The theory of Stockmayer in the modifications of Thurmond and Zimm has been used for the description of the size exclusion chromatography separation of randomly branched molecules with tetrafunctional branch points. It is assumed that free chain ends, created by the branching process, cause the molecules to be entrapped in the pores of the column packing with the time of their release given by the exponential law characteristic for the monomolecular reactions. Using this assumption, the anomalous elution behavior of such molecules can be modeled. With increasing elution volume, the average values of radius of gyration and, to a lesser degree, of molecular weight decrease and, after passing a minimum, again increase in the low-molecular weight region.
Permanent Link: http://hdl.handle.net/11104/0309736