Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes

0524608 - MBÚ 2021 RIV US eng J - Journal Article
Mylenko, Mykola - Vu, Dai Long - Kuta, Jan - Ranglová, Karolína - Kubáč, David - Lakatos, Gergely Erno - Grivalský, Tomáš - Caporgano, Martin Pablo - Manoel, Joao Camara - Kopecký, Jiří - Masojídek, Jiří - Hrouzek, Pavel
Selenium Incorporation to Amino Acids in Chlorella Cultures Grown in Phototrophic and Heterotrophic Regimes.
Journal of Agricultural and Food Chemistry. Roč. 68, č. 6 (2020), s. 1654-1665. ISSN 0021-8561. E-ISSN 1520-5118
R&D Projects: GA MŠMT(CZ) LO1416
EU Projects: European Commission(XE) 727874 - SABANA
Research Infrastructure: RECETOX II - 90051
Institutional support: RVO:61388971
Keywords : Chlorella * microalgae * selenium
OECD category: Agricultural biotechnology and food biotechnology
Impact factor: 5.279, year: 2020
Method of publishing: Limited access
https://pubs.acs.org/doi/full/10.1021/acs.jafc.9b06196

Microalgae accumulate bioavailable selenium-containing amino acids (Se-AAs), and these are useful as a food supplement. While this accumulation has been studied in phototrophic algal cultures, little data exists for heterotrophic cultures. We have determined the Se-AAs content, selenium/sulfur (Se/S) substitution rates, and overall Se accumulation balance in photo-and heterotrophic Chlorella cultures. Laboratory trials revealed that heterotrophic cultures tolerate Se doses, similar to 8-fold higher compared to phototrophic cultures, resulting in a similar to 2-3-fold higher Se-AAs content. In large-scale experiments, both cultivation regimes provided comparable Se-AAs content. Outdoor phototrophic cultures accumulated up to 400 mu g g(-1) of total Se-AAs and exhibited a high level of Se/S substitution (5-10%) with 30-60% organic/total Se embedded in the biomass. A slightly higher content of Se-AAs and ratio of Se/S substitution was obtained for a heterotrophic culture in pilot-scale fermentors. The data presented here shows that heterotrophic Chlorella cultures provide an alternative for Se-enriched biomass production and provides information on Se-AAs content and speciation in different cultivation regimes.
Permanent Link: http://hdl.handle.net/11104/0308950