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Global transcriptome analysis of porcine oocytes in correlation with follicle size

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    0523859 - ÚŽFG 2021 RIV US eng J - Journal Article
    Gad, Ahmed - Němcová, Lucie - Murín, Matěj - Kinterová, Veronika - Kaňka, Jiří - Laurinčík, Jozef - Benc, M. - Pendovski, L. - Procházka, Radek
    Global transcriptome analysis of porcine oocytes in correlation with follicle size.
    Molecular Reproduction and Development. Roč. 87, č. 1 (2020), s. 102-114. ISSN 1040-452X. E-ISSN 1098-2795
    R&D Projects: GA MŠMT EF15_003/0000460
    Institutional support: RVO:67985904
    Keywords : follicular size * oocyte * porcine * RNAseq
    OECD category: Developmental biology
    Impact factor: 2.609, year: 2020
    Method of publishing: Limited access
    https://asep.lib.cas.cz/arl-cav/cs/csg/?repo=crepo1&key=81409204677

    Although our knowledge regarding oocyte quality and development has improved significantly, the molecular mechanisms that regulate and determine oocyte developmental competence are still unclear. Therefore, the objective of this study was to identify and analyze the transcriptome profiles of porcine oocytes derived from large or small follicles using RNA high-throughput sequencing technology. RNA libraries were constructed from oocytes of large (LO 3-6 mm) or small (SO 1.5-1.9 mm) ovarian follicles and then sequenced in an Illumina HiSeq4000. Transcriptome analysis showed a total of 14,557 genes were commonly detected in both oocyte groups. Genes related to the cell cycle, oocyte meiosis, and quality were among the top highly expressed genes in both groups. Differential expression analysis revealed 60 up- and 262 downregulated genes in the LO compared with the SO group. BRCA2, GPLD1, ZP3, ND3, and ND4L were among the highly abundant and highly significant differentially expressed genes (DEGs). The ontological classification of DEGs indicated that protein processing in endoplasmic reticulum was the top enriched pathway. In addition, biological processes related to cell growth and signaling, gene expression regulations, cytoskeleton, and extracellular matrix organization were among the highly enriched processes. In conclusion, this study provides new insights into the global transcriptome changes and the abundance of specific transcripts in porcine oocytes in correlation with follicle size.
    Permanent Link: http://hdl.handle.net/11104/0308135

     
     
Number of the records: 1  

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