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MicroX-ray absorption near edge structure tomography reveals cell-specific changes of Zn ligands in leaves of turnip yellow mosaic virus infected plants
- 1.0511083 - BC 2020 RIV NL eng J - Journal Article
Mijovilovich, Ana - Mishra, Archana - Brueckner, D. - Spiers, K. - Andresen, Elisa - Garrevoet, J. - Falkenberg, G. - Küpper, Hendrik
MicroX-ray absorption near edge structure tomography reveals cell-specific changes of Zn ligands in leaves of turnip yellow mosaic virus infected plants.
Spectrochimica Acta Part B: Atomic Spectroscopy. Roč. 157, May (2019), s. 53-62. ISSN 0584-8547. E-ISSN 1873-3565
R&D Projects: GA MŠMT EF15_003/0000336
Institutional support: RVO:60077344
Keywords : Microspectroscopy * Microtomography * XANES * Zinc * Noccaea ochroleucum
OECD category: Biophysics
Impact factor: 3.086, year: 2019
Method of publishing: Open access
https://www.sciencedirect.com/science/article/pii/S0584854718305329?via%3Dihub
As many metals are essential for plants, excess or deficiency of them or alteration of their uptake, translocation, sequestration or physiological use can have severe consequences for plant growth and fitness. Therefore, investigating the distribution and speciation of metals in tissues is essential to understand plant physiology. We present a method based on non-destructive Synchrotron X-ray microtomography combined with microspectroscopy for studying metal distribution and speciation in plant tissues. By using the Maia detector system and the high flux of the undulator beam at the P06 beamline of the PETRA III synchrotron (at DESY), it was possible to record micro X-ray Absorption Near Edge Structure (mu XANES) for every voxel of a tomogram. The metal co-ordination in regions of interest within the tissue samples could be determined by comparing the XANES with spectra of relevant reference compounds. Metal distribution and coordination were measured in shock frozen hydrated plant leaves in a cryostream, avoiding sample preparation artefacts like liquid cell content redistribution that occurs with other preparation methods, unequal distribution of stains in staining assays, sample degradation by beam damage and thawing, etc. A spatial resolution of 5 mu m was selected, which is sufficient to resolve all leaf tissues (epidermis, palisade mesophyll, spongy mesophyll, veins), larger cells and biomineralization hotspots.
Permanent Link: http://hdl.handle.net/11104/0302013
Number of the records: 1