Acetonitrile-assisted enzymatic digestion can facilitate the Bottom-up identification of proteins of cancer origin

0501150 - ÚIACH 2020 RIV US eng J - Journal Article
Laštovičková, Markéta - Bobál, P. - Strouhalová, Dana - Bobálová, Janette
Acetonitrile-assisted enzymatic digestion can facilitate the Bottom-up identification of proteins of cancer origin.
Analytical Biochemistry. Roč. 570, APR (2019), s. 1-4. ISSN 0003-2697. E-ISSN 1096-0309
R&D Projects: GA MŠMT(CZ) 8F17003
Institutional support: RVO:68081715
Keywords : breast cancer * improvement of digestion * proteins
OECD category: Analytical chemistry
Impact factor: 2.877, year: 2019
Method of publishing: Limited access

In-gel trypsin digestion protocol using aqueous/acetonitrile solvent system facilitates MS analysis and maximize the number of identified proteins from biological samples. The procedure increased the number of matched peptides, and allowed the identification of more proteins from breast cancer cells with higher coverage than the common digestion protocol.
Permanent Link: http://hdl.handle.net/11104/0293142