Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes

0485763 - BFÚ 2018 RIV GB eng J - Journal Article
Puterova, Janka - Razumova, O. - Martínek, T. - Alexandrov, O. - Divashuk, M. - Kubát, Zdeněk - Hobza, Roman - Karlov, G. - Kejnovský, Eduard
Satellite DNA and Transposable Elements in Seabuckthorn (Hippophae rhamnoides), a Dioecious Plant with Small Y and Large X Chromosomes.
Genome Biology and Evolution. Roč. 9, č. 1 (2017), s. 197-212. ISSN 1759-6653. E-ISSN 1759-6653
R&D Projects: GA ČR GBP501/12/G090
Grant - others:GA MŠk(CZ) LM2010005
Institutional support: RVO:68081707
Keywords : sex-chromosomes * repetitive sequences * silene-latifolia
OECD category: Developmental biology
Impact factor: 3.940, year: 2017

Seabuckthorn (Hippophae rhamnoides) is a dioecious shrub commonly used in the pharmaceutical, cosmetic, and environmental industry as a source of oil, minerals and vitamins. In this study, we analyzed the transposable elements and satellites in its genome. We carried out Illumina DNA sequencing and reconstructed the main repetitive DNA sequences. For data analysis, we developed a new bioinformatics approach for advanced satellite DNA analysis and showed that about 25% of the genome consists of satellite DNA and about 24% is formed of transposable elements, dominated by Ty3/Gypsy and Ty1/Copia LTR retrotransposons. FISH mapping revealed X chromosome-accumulated, Y chromosome-specific or both sex chromosomes-accumulated satellites but most satellites were found on autosomes. Transposable elements were located mostly in the subtelomeres of all chromosomes. The 5S rDNA and 45S rDNA were localized on one autosomal locus each. Although we demonstrated the small size of the Y chromosome of the seabuckthorn and accumulated satellite DNA there, we were unable to estimate the age and extent of the Y chromosome degeneration. Analysis of dioecious relatives such as Shepherdia would shed more light on the evolution of these sex chromosomes.
Permanent Link: http://hdl.handle.net/11104/0280706