Structure of Psb29/Thf1 and its association with the FtsH protease complex involved in photosystem II repair in cyanobacteria

0484710 - MBÚ 2018 RIV GB eng J - Journal Article
Bečková, Martina - Yu, J.F. - Krynická, Vendula - Kozlo, A. - Shao, S.X. - Koník, Peter - Komenda, Josef - Murray, J. W. - Nixon, P. J.
Structure of Psb29/Thf1 and its association with the FtsH protease complex involved in photosystem II repair in cyanobacteria.
Philosophical Transactions of the Royal Society B-Biological Sciences. Roč. 372, č. 1730 (2017), č. článku 20160394. ISSN 0962-8436. E-ISSN 1471-2970
R&D Projects: GA ČR GBP501/12/G055; GA MŠMT(CZ) LO1416; GA MŠMT(CZ) ED2.1.00/19.0392
Institutional support: RVO:61388971
Keywords : photoinhibition * thylakoid formation 1 gene * D1 subunit
OECD category: Microbiology
Impact factor: 5.666, year: 2017

One strategy for enhancing photosynthesis in crop plants is to improve their ability to repair photosystem II (PSII) in response to irreversible damage by light. Despite the pivotal role of thylakoid-embedded FtsH protease complexes in the selective degradation of PSII subunits during repair, little is known about the factors involved in regulating FtsH expression. Here we show using the cyanobacterium Synechocystis sp. PCC 6803 that the Psb29 subunit, originally identified as a minor component of His-tagged PSII preparations, physically interacts with FtsH complexes in vivo and is required for normal accumulation of the FtsH2/FtsH3 hetero-oligomeric complex involved in PSII repair. We show using X-ray crystallography that Psb29 from Thermosynechococcus elongatus has a unique fold consisting of a helical bundle and an extended C-terminal helix and contains a highly conserved region that might be involved in binding to FtsH. A similar interaction is likely to occur in Arabidopsis chloroplasts between the Psb29 homologue, termed THF1, and the FTSH2/FTSH5 complex. The direct involvement of Psb29/THF1 in FtsH accumulation helps explain why THF1 is a target during the hypersensitive response in plants induced by pathogen infection. Downregulating FtsH function and the PSII repair cycle via THF1 would contribute to the production of reactive oxygen species, the loss of chloroplast function and cell death.
Permanent Link: http://hdl.handle.net/11104/0280078