Number of the records: 1
Flow and Aggregation of Rod-like Proteins in Slit and Cylindrical Pores Coated with Polymer Brushes: An Insight from Dissipative Particle Dynamics.
- 1.0471576 - ÚCHP 2017 RIV GB eng J - Journal Article
Posel, Zbyšek - Svoboda, Martin - Colina, C.M. - Lísal, Martin
Flow and Aggregation of Rod-like Proteins in Slit and Cylindrical Pores Coated with Polymer Brushes: An Insight from Dissipative Particle Dynamics.
Soft Matter. Roč. 13, č. 8 (2017), s. 1634-1645. ISSN 1744-683X. E-ISSN 1744-6848
R&D Projects: GA ČR GA13-09914S
Institutional support: RVO:67985858
Keywords : chains * dendrimers * glycoproteins
OECD category: Physical chemistry
Impact factor: 3.709, year: 2017
We use a meso-scale dissipative particle dynamics method to simulate the flow and aggregation of rodlike protein solutions through pores grafted with a solvent-sensitive polymer brush. The coated pores can control protein permeability and aggregation by a stretch-to-collapse conformational transition of the brush polymers in response to changes in the solvent quality. The protein solutions mimic aqueous glycoprotein solutions and proteins are represented as rod-like objects formed by coarse-grain beads.
The model further employs two types of beads to represent the existence of cystein-like terminal groups in real glycoproteins and mimic the aggregation of real glycoproteins in aqueous solutions. We vary the solvent quality with respect to the brush chains and study the flow and aggregation of rod-like proteins in the slit and cylindrical pores as the brush polymers undergo the stretch-to-collapse transition. The results show that stretched brush chains close the pore, hamper proteins’ flow and promote proteins’ aggregation. The collapsed brush chains open the pores for proteins’ flow and suppress their aggregation. Therefore, we observe more than a ten-fold reduction in the permeation rate of proteins in both pore geometries. Finally, due to pore confinement, larger proteins’ aggregates are formed in the slit pore than in the cylindrical pore, while more pronounced orientation of proteins in the flow direction is seen in the cylindrical pore than in the slit pore.
Permanent Link: http://hdl.handle.net/11104/0269527
File Download Size Commentary Version Access 0471576.pdf 0 7.6 MB Author’s postprint open-access
Number of the records: 1