Volatiles from spruce trap-trees detected by Ips typographus bark beetles: chemical and electrophysiological analyses

0430538 - ÚOCHB 2015 RIV NL eng J - Journal Article
Kalinová, Blanka - Břízová, Radka - Knížek, M. - Turčáni, M. - Hoskovec, Michal
Volatiles from spruce trap-trees detected by Ips typographus bark beetles: chemical and electrophysiological analyses.
Arthropod-Plant Interactions. Roč. 8, č. 4 (2014), s. 305-316. ISSN 1872-8855. E-ISSN 1872-8847
Institutional support: RVO:61388963
Keywords : Ips typographus * Picea abies * host selection * semiochemicals * GC-EAD * GC9GC/TOFMS
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 1.462, year: 2014

In the search for compounds that contribute to the host or habitat discrimination, antennae of Ips typographus were screened for sensitivity to volatiles released by spruce trap-trees using gas chromatography linked to electroantennography. The antennally active compounds were determined using comprehensive two-dimensional gas chromatography with time-of-flight mass spectrometric detection. Data show that I. typographus antennae respond to compounds emitted by the host. In total, 18 of antennally active compounds were detected: alpha-pinene, camphene, sabinene, beta-pinene, myrcene, Delta-3-carene, p-cymene, limonene, beta-phellandrene, 1,8-cineole, gamma-terpinene, terpinolene, nonanal, camphor, trans-pinocamphone, cis-pinocamphone, terpinen-4-ol, and verbenone. Unequivocal identification of all active minor compounds is provided and confirmed using synthetic standards. Compounds in minor quantities like 1,8-cineole, beta-phellandrene, camphor, cis-pinocamphone, and trans-pinocamphone were more active than major spruce monoterpenes. We hypothesize that the minor spruce compounds may play so far unrecognized role in conveying information about host suitability for I. typographus.
Permanent Link: http://hdl.handle.net/11104/0235572