Methods and apparatus for investigation of volume change dynamics of physiologically active samples

0424791 - BC 2014 RIV eng P - Patent Document
Roháček, Karel - Adámek, P. - Kloz, M. - Bína, David
Methods and apparatus for investigation of volume change dynamics of physiologically active samples.
2013. Owner: Biologické centrum AV ČR, v. v. i., Roháček Karel, Adamek Petr, Kloz Miroslav, Bína David. Date of the patent acceptance: 27.08.2013. Patent Number: US8520215
R&D Projects: GA AV ČR IAA600960716
Institutional support: RVO:60077344
Keywords : Volume change dynamics * Interferometric device * Physiological samples
Subject RIV: BO - Biophysics
http://patft.uspto.gov/netacgi/nph-Parser?Sect1=PTO1&Sect2=HITOFF&d=PALL&p=1&u=%2Fnetahtml%2FPTO%2Fsrchnum.htm&r=1&f=G&l=50&s1=8520215.PN.&OS=PN/8520215&RS=PN/8520215

The volume change dynamics of photosynthetically active samples, e.g. plants, algae, bacteria, have been examined so far by means of the photoacoustic method, which does not enable dynamically accurate measurement of transversal changes of these samples in real time in vivo. According to the invention, the transversal change of a position of a measured sample surface exposed to a visible radiation is measured interferometrically using a reflective target, which is in mechanical contact with the sample surface, and reflects a measuring beam generated together with a reference beam by laser. The transversal change of the measured sample surface is detected from the phase difference of harmonic waves in the beam of interfering radiation. The novel device involves a special detection connection of photodetectors, a measuring unit and a basic module with a computer, the design of reflective targets for liquid and solid samples, an optomechanical mechanism and a holder of solid samples.
Permanent Link: http://hdl.handle.net/11104/0230807