Biosensing enhancement using passive mixing structures for microarray-based sensors

0421853 - ÚFE 2015 RIV GB eng J - Journal Article
Lynn, Nicholas Scott - Martínez-López, J.I. - Bocková, Markéta - Adam, Pavel - Coello, V. - Siller, R.H. - Homola, Jiří
Biosensing enhancement using passive mixing structures for microarray-based sensors.
Biosensors and Bioelectronics. Roč. 54, 15 April (2014), s. 506-514. ISSN 0956-5663. E-ISSN 1873-4235
R&D Projects: GA ČR GBP205/12/G118
Institutional support: RVO:67985882
Keywords : Microfluidic mixing * Biosensors * Microfluidics
Subject RIV: BO - Biophysics
Impact factor: 6.409, year: 2014

The combination of microarray technologies with microfluidic sample delivery and real-time detection methods has the capability to simultaneously monitor 10–1000 s of biomolecular interactions in a single experiment. Despite the benefits that microfluidic systems provide, they typically operate in the laminar flow regime under mass transfer limitations, where large analyte depletion layers act as a resistance to analyte capture. By locally stirring the fluid and delivering fresh analyte to the capture spot, the use of passive mixing structures in a microarray environment can reduce the negative effects of these depletion layers and enhance the sensor performance. Despite their large potential, little attention has been given to the integration of these mixing structures in microarray sensing environments. In this study, we use passive mixing structures to enhance the mass transfer of analyte to a capture spot within a microfluidic flow cell. Using numerical methods, different structure shapes and heights were evaluated as means to increase local fluid velocities, and in turn, rates of mass transfer to a capture spot. These results were verified experimentally via the real-time detection of 20-mer ssDNA for an array of microspots.
Permanent Link: http://hdl.handle.net/11104/0228105