Senzitive method of Caspase-3 detection in single stem cell

0420849 - ÚIACH 2014 RIV CZ eng C - Conference Paper (international conference)
Adamová, Eva - Klepárník, Karel - Matalová, Eva
Senzitive method of Caspase-3 detection in single stem cell.
Chemické Listy. Roč. 107, Issue s3.. Praha: Česká společnost chemická, 2013, s. 289-290. ISSN 0009-2770. E-ISSN 1213-7103.
[CECE Junior 2013. Brno (CZ), 12.11.2013-13.11.2013]
R&D Projects: GA ČR GAP206/11/2377; GA MŠMT(CZ) EE2.3.20.0182
Institutional support: RVO:68081715 ; RVO:67985904
Keywords : caspase-3 * single stem cell * miniaturization
Subject RIV: CB - Analytical Chemistry, Separation; EB - Genetics ; Molecular Biology (UZFG-Y)

Caspases are involved in physiological process (e.g. cellular differentiation) but also can cause serious disorders. It is getting necessary to develop sensitive, miniaturized and fast methods amenable to analyze small amounts of samples. Luciferin/luciferase chemiluminescence reaction is one of the methods of caspase-3 detection. We have developed a miniaturized device enabling detection of caspase-3 and quantitation just in femtogram level (10–15 g) in single apoptotic human stem cells (neural crest derived). The technology is based on the specific cleavage of modified luciferin by caspase-3, emissions of photons and their detection by photomultiplier tube working in the photon counting regime.
Permanent Link: http://hdl.handle.net/11104/0227392