Proteomic analysis of sperm estrogen response

0380444 - BTÚ 2013 CZ eng A - Abstract
Šebková, N. - Černá, M. - Děd, Lukáš - Pěknicová, Jana - Hortová, K.
Proteomic analysis of sperm estrogen response.
XVII. symposium českých reprodukčních imunologů s mezinárodní účastí. Praha: Biotechnologický ústav v. v. i. AVČR, 2011 - (Pěknicová, J.). s. 53-53
[XVII. symposium českých reprodukčních imunologů s mezinárodní účastí. 26.05.2011-29.05.2011, Žďár nad Sázavou]
R&D Projects: GA MŠMT(CZ) 1M06011
Institutional research plan: CEZ:AV0Z50520701
Keywords : proteomic analysis * estrogens * capacitation * acrosomal reaction
Subject RIV: DN - Health Impact of the Environment Quality

Steroidal hormones estrogens play an essential role in the development of male germ cells during spermatogenesis and while sperm gaining fertilizing ability. It has been well reported that environmental estrogens can interfere with function of endogenous hormones in very low concentrations. They can exert a specific response in cells by interacting with specific receptors activating several signaling pathways, through which they display false hormone-like activity. Tyrosine phosphorylation of several proteins is one of the markers of a properly ongoing process of capacitation followed by a fusion of plasma and outer acrosomal membrane and exocytosis of the acrosomal vesicle. The present study addressed a question, whether a rising concentration of natural estrogens and synthetical estrogenic compounds in the environment can modify mouse sperm capacitation or acrosome reaction in vitro. The effect of estrogenes were evaluated during capacitation in vitro in the sperm head by fluorescent markers as well as by electrophoresis of sperm cell lysate. Simultaneously, we evaluated the state of the acrosome after calcium ionophore induced acrosomal reaction. Generally, estrogens showed to stimulate p-Tyr in sperm during capacitation in vitro depending on their concentration. The number of sperm capable of undergoing head p-Tyr as well as the overall p-Tyr was generally significantly higher when compared to the control. In contrast, the number of sperm that underwent the CaI induced AR was lower in experimental groups. In conclusion, this study has provided the evidence that estrogens significantly stimulate capacitation progress in the concentration-dependent manner. On the other hand, estrogens decrease number of acrosome reacted sperm after the induced AR. Based on our results, it can be inferred that raising a concentration of estrogens in the environment may represent a potential risk in altering certain mechanisms contributing to the fertilizing capability of sperm.
Permanent Link: http://hdl.handle.net/11104/0211155