Carboxylated calixarenes bind strongly to CD69 and protect CD69+ killer cells from suicidal cell death induced by tumor cell surface ligands

0350689 - MBÚ 2011 RIV GB eng J - Journal Article
Bezouška, K. - Šnajdrová, R. - Křenek, K. - Vančurová, Markéta - Kádek, A. - Adámek, D. - Lhoták, P. - Kavan, Daniel - Hofbauerová, Kateřina - Man, Petr - Bojarová, Pavla - Křen, Vladimír
Carboxylated calixarenes bind strongly to CD69 and protect CD69+ killer cells from suicidal cell death induced by tumor cell surface ligands.
Bioorganic & Medicinal Chemistry. Roč. 18, č. 4 (2010), s. 1434-1440. ISSN 0968-0896. E-ISSN 1464-3391
R&D Projects: GA MŠMT OC 136; GA MŠMT 1M0505; GA ČR GA303/09/0477; GA ČR GD305/09/H008; GA AV ČR IAA400200503; GA AV ČR KJB500200612
Institutional research plan: CEZ:AV0Z50200510
Keywords : Carboxylated calixarenes * Lymphocyte activation * Receptor cross-linking
Subject RIV: CE - Biochemistry
Impact factor: 2.978, year: 2010

We have recently identified a new class of high affinity ligands for CD69 leukocyte membrane receptor, carboxylated calixarenes. Of the three compounds investigated here, thiacalix[4]arene had the highest affinity for CD69 in direct binding assays, and proved to be the most specific inhibitor of CD69 identified so far in receptor precipitation and cellular activation experiments. Carboxylated calixarenes also proved effective at protection of CD69(high) lymphocytes from apoptosis triggered by a multivalent ligand or antibody. Thus, carboxylated calixarenes set a new paradigm for noncarbohydrate ligands for CD69 making them attractive for protection of killer cells in combined animal tumor therapies
Permanent Link: http://hdl.handle.net/11104/0190625