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Selective binding of tumor suppressor p53 protein to topologically constrained DNA: Modulation by intercalative drugs
- 1.0342523 - BFÚ 2011 RIV NL eng J - Journal Article
Pivoňková, Hana - Šebest, Peter - Pečinka, P. - Tichá, Olga - Němcová, Kateřina - Brázdová, Marie - Brázdová Jagelská, Eva - Brázda, Václav - Fojta, Miroslav
Selective binding of tumor suppressor p53 protein to topologically constrained DNA: Modulation by intercalative drugs.
Biochemical and Biophysical Research Communications. Roč. 393, č. 4 (2010), s. 894-899. ISSN 0006-291X. E-ISSN 1090-2104
R&D Projects: GA AV ČR(CZ) IAA500040701; GA ČR(CZ) GP204/07/P476; GA ČR(CZ) GP301/07/P160; GA AV ČR(CZ) 1QS500040581; GA MŠMT(CZ) LC06035; GA ČR(CZ) GA204/08/1560
Institutional research plan: CEZ:AV0Z50040507; CEZ:AV0Z50040702
Keywords : p53-DNA binding * supercoiled DNA * DNA topology
Subject RIV: BO - Biophysics
Impact factor: 2.595, year: 2010
Selective binding of p53 to negatively and positively supercoiled DNA was studied using intercalative drugs chloroquine, ethidium bromide, acridine derivatives and doxorubicin as a modulators of the level of DNA supercoiling. The p53 was found to lose gradually its preferential binding to negatively scDNA with increasing concentrations of intercalators until the DNA negative superhelix turns were relaxed. Formation of positive superhelices rendered the circular duplex DNA to be preferentially bound by the p53 again.
Permanent Link: http://hdl.handle.net/11104/0185238
Number of the records: 1