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Extraction and separation of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species by CZE
- 1.0336576 - ÚOCHB 2010 RIV DE eng J - Journal Article
Sázelová, Petra - Kašička, Václav - Ibanez, E. - Cifuentes, A.
Extraction and separation of water soluble proteins from Bacillus thuringiensis-transgenic and non-transgenic maize species by CZE.
Journal of Separation Science. Roč. 32, č. 21 (2009), s. 3801-3808. ISSN 1615-9306. E-ISSN 1615-9314
R&D Projects: GA ČR(CZ) GA203/08/1428
Grant - others:GA ČR(CZ) GA203/09/0675
Program: GA
Institutional research plan: CEZ:AV0Z40550506
Keywords : Bacillus thuringiensis-transgenic maize * CZE-UV profiling * Maize proteins
Subject RIV: CB - Analytical Chemistry, Separation
Impact factor: 2.551, year: 2009
A new method for extraction and capillary electrophoretic (CE) separation and characterization of water-soluble proteins (albumin fraction) from Bacillus thuringiensis-transgenic and non-transgenic maize varieties was developed. The best CE separation of extracted proteins was achieved in isoelectric background electrolyte (200mM iminodiacetic acid, pH 2.26). Well resolved components of protein extracts were characterized by effective electrophoretic mobilities and corrected peak areas. Significant qualitative and quantitative differences in CE-UV profiling were found, which could potentially be used to distinguish the B. thuringiensis variety from the wild maize species.
Permanent Link: http://hdl.handle.net/11104/0180780
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