Polymorphism in the porcine NGFB gene detected by DGGE and its linkage mapping

0213255 - UZFG-Y 20003043 RIV CZ eng J - Journal Article
Kopečný, Michal - Stratil, Antonín - Čepica, Stanislav - Moser, G.
Polymorphism in the porcine NGFB gene detected by DGGE and its linkage mapping.
Czech Journal of Animal Science. Roč. 45, č. 4 (2000), s. 189-192. ISSN 1212-1819. E-ISSN 1805-9309
R&D Projects: GA ČR GA523/97/1305; GA AV ČR KSK2052601
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 0.172, year: 2000

The porcine nerve growth factor beta gene (NGFB) belongs to a conserved syntenic group on chromosome 4. The objective of this study was to Find a simply detectable polymorphism and use it in genetic studies. The new set of PCR primers was used to amplify a 448 bp Fragment of the gene. Polymorphism was studied by denaturing gradient gel electrophoresis (DGGE). One hundred and fifty-four pigs belonging to eight breeds were studied and two alleles, A and B, were detected. Frequencies of B allele were: Large White - 0.50, Landrace - 0.96, Pietrain - 0.81 and Black Pied Prestice - 0.83. Czech Meat Pig (14 pigs), Hampshire (6), Duroc (2) and Meishan (8) were monomorphic for allele B. Data from three Hohenheim PiGMaP F-2 pedigrees were used for linkage mapping. The sex average locations of the NGFB gene were tin Kosambi cM) 101.8 (Wild Boar x Pietrain), 95.5 (Meishan x Pietrain) and 89.5 (Wild Boar x Meishan), respectively.
Permanent Link: http://hdl.handle.net/11104/0108793