Bovine seminal ribonuclease attached to nanoparticles made of polylactic acid kills leukemia and lymphoma cell lines in vitro

0213236 - UZFG-Y 20003024 RIV US eng J - Journal Article
Michaells, M. - Matoušek, Josef - Vogel, J. U. - Slavík, Tomáš - Langer, K. - Činátl, J. - Kreuter, J. - Shwabe, D. - Činátl jr., J.
Bovine seminal ribonuclease attached to nanoparticles made of polylactic acid kills leukemia and lymphoma cell lines in vitro.
Anti-cancer Drugs. Roč. 11, - (2000), s. 369-376. ISSN 0959-4973. E-ISSN 1473-5741
R&D Projects: GA AV ČR KSK2052601
Subject RIV: EB - Genetics ; Molecular Biology
Impact factor: 1.570, year: 2000

Bovine seminal ribonuclease (BS-RNase) is a protein with a number of biological effects. It shows antitumoral, aspermatogenic, antiembryonic, immunosuppressive and antiviral properties. The cytotoxic effects appear to be specific for tumor cells as non-malignant cells seem to be unaffected in vitro. Unfortunately, the in vivo application of BS-RNase so far was successful only when it was administered intratumorally. Therefore, the objective of the present investigation was to improve the properties of BS-RNase by attachment to nanoparticles made of polylactic acid (PLA-NP) using an adsorption method. This preparation was tested in vitro against leukemia (MOLT-4) and lymphoma (H9) cell lines sensitive and resistant to cytarabine. No difference between the nanoparticle preparation and pure BS-RNase was found in these tests, To examine the in vivo effects, the preparations were tested for their aspermatogenic and antiembryonal efficacy compared to the pure BS-RNase as a rapid test for antitumoral activity. The aspermatogenic and antiembryonal effects were enhanced by the nanoparticle preparation, Consequently, BS-RNase loaded adsorptively to PLA-NP holds promise for the in vivo use as an antitumoral agent. Further research will investigate the efficacy of this preparations in an in vivo tumor model. [(C) 2000 Lippincott Williams & Wilkins.].
Permanent Link: http://hdl.handle.net/11104/0108774